Fig. 4

Colocalization of RAB3A with KIF1A variants that carry ALS-associated mutations. a Cultured mouse cortical neurons (DIV7) were cotransfected with the RFP-RAB3A construct, along with the Venus-tagged WT and mutant KIF1A constructs. Neurons were fixed and imaged at DIV11. Scale bar, 5 μm. b Quantitative analysis of the percentage of RAB3A colocalized with KIF1A in a. n = 24–39 neurons from three independent experiments. *P < 0.05, ****P < 0.0001; P = 0.0659 (WT vs R1100C); P < 0.0001 (WT vs R1356Q); P = 0.201 (WT vs P1587L); P = 0.0104 (WT vs A1643V) by one-way ANOVA and Fisher’s test. Error bars represent SEM