Fig. 2

RT-QuIC detection of α-synuclein seeding activity in the nasal swabs (NS) of PD patients at agger nasi (AN) and middle turbinate (MT). a The final average relative ThT fluorescence from four replicate readings obtained from NS of each individual case (n = 23) at the level of AN and through the MT at 80 h. Bars show the average ± SD for all the cases in each group. The dashed line shows the fluorescence threshold for a positive result. b Traces represent the relative average percentage of ThT fluorescence readings of positive samples from AN (blue trace) and the MT (magenta trace), and non-PD (grey trace) as negative controls. The means (thick lines) with SDs (thin lines) of those averages are shown as a function of RT-QuIC reaction time