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Fig. 9 | Translational Neurodegeneration

Fig. 9

From: Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus–response in spinal muscular atrophy

Fig. 9

Total and surface TrkB levels are not altered in growth cones of Smn-deficient motoneurons. a Representative Western blot of total protein lysates obtained from cultured Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg neurons probed against TrkB. GAPDH was used as loading control. b Representative images of soma of Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg motoneurons that were immunostained against TrkB. c Quantification of TrkB signal intensities in the soma shows similar TrkB levels in both Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg motoneurons (n.s., P = 0.454; n = 81–85 cells from 4 independent experiments). d Representative images of Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg motoneurons showing total TrkB levels in the growth cone. e Relatively higher TrkB levels/area in growth cones of Smn−/−;SMN2tgtg compared to Smn+/+;SMN2tgtg neurons (n.s., P = 0.0512; n = 71–74 cells from 4 independent experiments). f Motoneurons from Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg were labeled against surface presented TrkB in the growth cone. Synaptophysin antibody was used to label growth cone boundaries. g Graph shows that the surface TrkB levels are comparable in Smn+/+;SMN2tgtg versus Smn−/−;SMN2tgtg neurons (n.s., P = 0.999; n = 70 cells from 3 independent experiments). All data are normalized to Smn+/+;SMN2tgtg control. Data are presented in scatter dot plot; error bars represent mean ± SEM. Statistical analyses were done by two-tailed Mann Whitney test

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