Fig. 3

BDNF stimulation does not trigger rapid ribosomal redistribution and protein synthesis in growth cones of Smn-deficient motoneurons. a Representative images of growth cones from Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg motoneurons immunostained against rRNA using Y10B antibody. b Quantification of Y10B mean intensities shows that in contrast to Smn+/+;SMN2tgtg (****P < 0.0001; n = 52–214 cells from 3–5 independent experiments), the levels of Y10B do not increase in growth cones of BDNF-stimulated Smn−/−;SMN2tgtg motoneurons (n.s., P = 0.0774; n = 51–202 cells from 3–5 independent experiments). c Representative images of growth cones of Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg motoneurons immunostained against puromycin after BDNF pulse application. d Puromycin immunoreactivity does not increase in BDNF-stimulated Smn−/−;SMN2tgtg (n.s., P > 0.9999; n = 61–83 cells from 3 independent experiments) compared to Smn+/+;SMN2tgtg neurons (**P = 0.0061; ****P < 0.0001; n = 32–39 cells from 2 independent experiments). e Representative images of growth cones of Smn+/+;SMN2tgtg and Smn−/−;SMN2tgtg motoneurons stained against Cav2.2 (cyan) and Tau (red) after 1-min BDNF stimulation. f Immunoreactivity of Cav2.2 does not increase in BDNF-stimulated Smn−/−;SMN2tgtg (n.s., P > 0.9999; n = 64–66 cells from 3 independent experiments) compared to Smn+/+;SMN2tgtg motoneurons (****P < 0.0001; n = 62–64 cells from 3 independent experiments). Data are presented in scatter dot plot; error bars represent mean ± SEM. Statistical analyses were done by One-way ANOVA with Dunn’s post-hoc test