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Fig. 5 | Translational Neurodegeneration

Fig. 5

From: Melatonin ameliorates tau-related pathology via the miR-504-3p and CDK5 axis in Alzheimer’s disease

Fig. 5

Melatonin reduces tau phosphorylation and the formation of sarkosyl-insoluble tau aggregates in 13-month-old hTau mice. Cortical and hippocampal tissues from WT (n = 8), hTau + SA (n = 5) and hTau + MT mice (n = 5) were separated into sarkosyl-soluble (a–d) and sarkosyl-insoluble fractions (e–h). The protein samples were subjected to immunoblotting analysis with anti-pThr231 Tau, anti-pSer262 Tau, anti-pSer396 Tau, anti-tau or anti-β-actin antibodies. Sarkosyl-soluble β-actin was used as an internal control and for normalization in both the sarkosyl-soluble and -insoluble fractions. Data are presented as the means ± standard errors (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; N.S., no significance)

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