Skip to main content
Fig. 6 | Translational Neurodegeneration

Fig. 6

From: α-Synuclein arginylation in the human brain

Fig. 6

E46 and E83 arginylation decreases intracellular α-syn aggregation. Mouse neurons were incubated with unmodified (WT) α-syn or α-syn arginylated at E46, E83, or both E46/E83 (double), and the formation of LB-like inclusions was examined with S129 antibody. a Quantification of the intensity, length, and width of S129-positive inclusions in neuronal cultures, plotted as mean with 95% confidence interval. n = 50 independent fields of view. P values were calculated by unpaired two-tailed Student's t-test, **P < 0.01, ***P < 0.001, ****P < 0.0001. See Additional file 1: Fig. S6 for scatter plots of the data shown in the left panels, plotted as individual data points correlated to relative measurements of the same aggregates. b Representative images of inclusions revealed by S129 staining. Scale bar, 10 µm. See Additional file 1: Fig. S7 for broader-field-of-view images of the same aggregates.  Images were processed by uniform background subtraction using Metamorph imaging software

Back to article page