Fig. 5

E46 and E83 arginylation prevents S129 phosphorylation. Representative Western blot images (left) and quantification (right) of phosphorylation of unmodified (U) and arginylated α-syn protein standards after the phosphorylation reaction with the addition of components shown on top. a α-Syn variants, either unmodified (U) or 100% arginylated at E46 or E83, were incubated with PLK2 kinase in the presence or absence of ATP. Arginylation greatly reduced the efficiency of PLK2 phosphorylation. b Phosphorylation of α-syn variants, either unmodified or arginylated at varied percentages as indicated, incubated with or without PLK2 kinase (PLK) as indicated on top. Arginylation interfered with PLK2 phosphorylation in a dose-dependent manner. Band intensities of S129 signal normalized to α-syn load are plotted. Error bars represent SEM, n = 3