Fig. 6

Hyperglycemia promotes neuroinflammation in BAC-α-syn-GFP mice. (a) Representative Western blots of Iba-1 and GFAP. (b, c) Quantification of Iba-1 and GFAP protein levels by intensity measurement (n = 3 to 4 mice/group). (d) Representative immunohistochemical Iba-1 staining of the SN of mice. (e) Number of SN microglia at each time point. (f) The morphology of SN microglia at four different activation stages. (g) The proportion of four types of microglia based on different morphology. WT + vehicle (n = 4/4 mice at one/three months after injection, respectively); WT + STZ (n = 4/4 mice at one/three months after injection, respectively); α-syn + vehicle (n = 3/4 mice at one/three months after injection, respectively); α-syn + STZ (n = 3/4 mice at one/three months after injection, respectively). Scale bars: (d), left column, 100 µm; right column, 50 µm; (f), 20 µm. *P < 0.05, **P < 0.01 in (b, c, e). *P < 0.05, **P < 0.01 α-syn + STZ versus α-syn + vehicle in stage IV at one and three months after injection (g)