Fig. 5

HFD causes impairments in  LTD and novel object location/recognition memory. a Schematic illustration of the timeline and LTD induction protocol by low-frequency stimulation (LFS) in acute CA1 hippocampal slices. b, d Field EPSP recordings in CA1 in acute slices from wt fed with a RD or HFD show no differences between the groups. c, d LTD induced in slices from heterozygous TBA2.1 mice fed with HFD, shows significant impairment when compared to wild-type (+/+) mice and TBA2.1 (+ /Tg) mice fed with RD. d Averaged fEPSP slope values measured 180–210 min after LTD induction. Data were obtained from slices prepared from at least three different mice. n = 15 for group 1; n = 13 for group 2; n = 9 for group 3; n = 12 for group 4. e Schematic representation of the object location and recognition memory experimental protocol. Scatter dot plot shows the total distance that wild-type and heterozygous TBA2.1 animals fed with a RD or HFD walked during the habituation phase (f), the discrimination index of the new located (NLR, g) or replaced object (NOR, h) as compared to the old one. In f n = 9 animals in groups 1 and 4; n = 11 in group 2; n = 8 in group 3. In g n = 12 for group 1; n = 14 for group 2; n = 11 for group 3; n = 10 for group 4. In h n = 12 for group 1; n = 14 for group 2; n = 11 for group 3; n = 9 for group 4. ***P < 0.001, **P < 0.01, *P < 0.05 versus control by two-way ANOVA followed by Bonferroni’s post-hoc test, n.s. = non-significant