Fig. 2

Perls’ Prussian blue staining to detect mouse brain damage. (a) Representative images of Perl’s Prussian blue staining in each mouse group. Blue pigments in the cortex indicate hemosiderins. No hemosiderin-positive deposit was found in the hippocampus. Scale bar, 100 μm. (b) Numbers of mice with or without hemosiderin deposits (P = 0.455 by Fisher’s exact test). (c) Numbers of hemosiderin deposits per section compared between the treatment groups. Box plots show medians and confidential intervals of 95% (P = 0.064 by Mann–Whitney U test; n = 6 for each group)