Fig. 4
From: Dense core vesicle markers in CSF and cortical tissues of patients with Alzheimer’s disease
DCV proteins are accumulated in GVD bodies in CA1 pyramidal neurons of AD patients. a Peroxidase immunolabelling shows PC2, PC1/3 and CPE accumulations in aberrant granules (arrowheads) located in CA1 pyramidal neurons of AD hippocampus. Scale bars, 10 μm. b PC2, PC1/3 and CPE colocalization with the markers of GVD bodies CK1δ and CHMP2B (arrows) and the presence of lumenal PC2 and the transmembrane LAMP1 in the same AD granule (open-arrows) in different neuronal cell bodies of AD cases. Scale bars, 2 μm. c PC2+ GVD inclusions are found either in mature (AT8++) (cell at left) or in undeveloped (AT8+) (cell at right) neurofibrillary tangles in CA1 pyramidal neurons of an AD case. Scale bar, 7 μm. d Graphs showing the percentages of cells displaying absent (0), few (< 10), or many (≥ 10) PC2- and PC1/3-labelled GVD in AD cases, depending on the occurrence of neurofibrillary tangles (negligible, AT8−; low, AT8+, strong, AT8++)