Fig. 3

The degenerating axons and spheroids accumulate DAergic debris. a A thick degenerating axon between normal thin axons of the MFB. b, c Accumulation of Vmat2 and APP respectively in the thick axon but not in the thin axons. d The fluorescence values under the white lines of a–c (mean ± standard error). f, g Vmat2 and APP immunoreactivity in the spheroid shown in e. h The mean ± standard error of fluorescence values indicated by the white lines in e–g. j, k Vmat2 and APP immunoreactivity of the two spheroids that were being formed in i by the fracture of a DAergic axon. l The mean ± standard error of the fluorescence values indicated by the white lines in e–g. n, o Vmat2 and αsyn immunoreactivity of the thick degenerating axon shown in m. p The mean ± standard error of the fluorescence values indicated by the white lines in m–o. r The GDNF immunoreactivity of the spheroid shown in q. t The GDNF immunoreactivity of the spheroid shown in s. u The mean ± standard error of the fluorescence values indicated by the white lines in q and r