Fig. 1

Selective degeneration of DAergic terminals in the striatum. a–f An example of the effect of increasing doses of 6-OHDA administered in the lateral ventricle 5 days before the study. g An example showing that the highest 6-OHDA dose (100 µg) induces astrocytosis and microgliosis in both the ipsilateral (g top) and contralateral (g bottom) striatum. The 25 µg dose denervated the nearby tissue of the ipsilateral but not of the contralateral striatum (c vs d, h, i), inducing local astrocytosis (j, k), with no change in the number of microglial cells (Iba1 in l) and with no macrophage activation (CD68 in m). The DAergic denervation was accompanied by thickening of axons, which facilitated the formation of spheroids (n). o A thickened axon whose membrane was marked with a DAT antibody