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Fig. 4 | Translational Neurodegeneration

Fig. 4

From: NMDAR-dependent somatic potentiation of synaptic inputs is correlated with β amyloid-mediated neuronal hyperactivity

Fig. 4

Somatic amplification of dendritic inputs depends on the activity of GluN2B-NMDARs located at the soma. a Illustration of stimulating and recording paradigm. Note that the MK-801 was either locally applied to the soma or to the dendrite of the recorded neuron where the stimulating electrode was placed (200 μm distance from the soma). b Sample traces showing averaged EPSPs recorded at the soma or at dendrites in hippocampal CA1 pyramidal neurons. Sample EPSP traces are the average of 30 consecutive events recorded at the time marked by the numbers (1 or 2) from c-f. MK-801 (50 μM) was locally applied with TFB-TBOA (100 nM) either at the soma or at the dendrite. c–f MK-801 applied at the soma totally abolished the TBOA-elicited EPSP potentiation. c, e Results from a single experiment displaying the time course and magnitude of the normalized EPSPs. d, f Summary of results from all experiments as that in (c) and (e), respectively. MK-801 applied to the dendrite failed to affect the TBOA-induced potentiation in normalized EPSPs, while MK-801 applied to the soma reversed the EPSP potentiation induced by TBOA. g Sample traces showing the effect of suppressing GluN2B- or Glu2NA-NMDARs on the average EPSPs recorded at the soma of hippocampal CA1 pyramidal neurons treated with TFB-TBOA (100 nM). Note that the antagonist of GluN2-NMDARs was locally applied to the soma. h–k Antagonist of GluN2B-NMDARs, but not of GluN2A-NMDARs applied at the soma, totally abolished the TBOA-elicited EPSP potentiation. Results from a single experiment (h, j) and summarized results (i, k) displaying the time course and the magnitude of the normalized EPSPs. l Statistical histogram showing the effect of locally applied NMDAR antagonists. MK-801 (50 μM) or ifenprodil (3 μM) applied to the soma totally reversed the TBOA-induced EPSP potentiation. Data are means ± SEM, t test, **P < 0.01

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