Fig. 1

TBOA changes the membrane property and induces neuronal hyperactivity. a, b The time course and magnitude of changes in RMP (a) and input resistance (b) after TFB-TBOA (100 nM) treatment. Note that the input resistance was measured by a single − 20 pA injection. The blue-shaded area indicates the time period of TFB-TBOA treatment. c Examples of AP firing triggered by a 600-ms current injection of either 30, 50 or 70 pA before (top) and 20 min after (bottom) TFB-TBOA (100 nM) treatment. d Summary data showing significant increase in the number of APs elicited by somatic current injection 20 min after TFB-TBOA (100 nM) treatment. e Example traces showing spontaneous firing of recorded CA1 pyramidal neurons before (RMP − 66.94 mV) and after (RMP was − 59.17 mV) TFB-TBOA treatment. AP number was dramatically increased. (f) Cumulative distribution of inter-spike intervals (ISIs). g Statistical histogram showing significant enhancement of firing rate after TFB-TBOA treatment. Data are means ± SEM; t test for pre- versus post-treatment comparison; *P < 0.05; **P < 0.01