Fig. 4From: Novel multivalent design of a monoclonal antibody improves binding strength to soluble aggregates of amyloid betaLigandTracer experiments illustrating the binding strength of the recombinant antibodies to Aβ1-42 protofibrils. Detection signal of iodine-125-labelled RmAb158 (blue), Tetra-RmAb158 (black) and Hexa-RmAb158 (red) to Aβ1-42 protofibrils, normalized to 100% corresponding to the maximum binding level, for evaluation of binding behavior and kinetics. Binding curves show association at 300 pM (antibody added at time point 0.5 h) and 1 nM (antibody added at time point 3 h), and dissociation (when the antibody was removed). Comparison of the affinity (KD), association rate constant (ka) and dissociation rate constant (kd) among the antibodies using one-to-one kinetic model. Results are generated from two repetitive experiments and average affinity constants are shownBack to article page