Fig. 3
PINK1 does not localize to mitochondria upon CCCP treatment in VPS35D620N cells. a Representative fluorescence images of WT or VPS35D620N (clone 1) cells treated with 10 μM or 20 μM CCCP for 24 h. From left to right: endogenous TOM20 staining; endogenous PINK1 staining; overlay image including DAPI staining for nuclei in which cells with co-localization (white) of TOM20 (magenta) and PINK1 (green) are outlined; zoom-in of highlighted area in overlay image. Scale bar, 10 μm, or 4 μm for zoomed images. b Quantification of co-localization of endogenous TOM20 and PINK1 of WT and VPS35D620N (clones 1 and 2) cells treated with 10 μM or 20 μM CCCP for 24 h. Each dot represents the Pearson coefficient calculated for one cell. Red lines show the median Pearson coefficient per cell line. Statistical analyses were performed using the Kruskal-Wallis test followed by a pairwise Mann-Whitney U-test with Benjamini-Hochberg multiple testing correction. ***P < 0.005, ****P < 0.001