Table 2 Primary and secondary antibodies in immunostaining
Source | Vender | Catalog No. | Dilution | Application | Type | |
|---|---|---|---|---|---|---|
anti-pSer129 | Rabbit | Abcam | ab51253 | 1:10,000 | Immunohistochemistry | 1st antibody |
1:1000 | Immunofluorescence | |||||
anti-Iba1 | Rabbit | Wako | 019–19,741 | 1:500 | Immunohistochemistry | 1st antibody |
anti-Iba1 | Mouse | Millipore | MABN92 | 1:1000 | Immunofluorescence | 1st antibody |
anti-ChAT | Goat | Millipore | AB144P | 1:100 | Immunohistochemistry | 1st antibody |
Biotinylated anti-rabbit | Goat | Vector Laboratories | BA-1000 | 1:500 | Immunohistochemistry | 2nd antibody |
Biotinylated anti-goat | Horse | Vector Laboratories | BA-9500 | 1:500 | Immunohistochemistry | 2nd antibody |
Cy3 anti-rabbit | Goat | Jackson Immuno Research | 139,290 | 1:500 | Immunofluorescence | 2nd antibody |
Cy3 anti-mouse | Donkey | Jackson Immuno Research | 715–165-150 | 1:200 | Immunofluorescence | 2nd antibody |
Alexa Fluor® 647 anti-rabbit | Donkey | Jackson Immuno Research | 711–605-152 | 1:200 | Immunofluorescence | 2nd antibody |