Fig. 5
From: Disruption of orbitofrontal-hypothalamic projections in a murine ALS model and in human patients
Altered cortico-hypothalamic projection pattern in kiFUS mice at P255. a Representative frontal brain sections of WT and kiFUS mice depicting projections from MOs (significantly decreased, arrow), PL + ORBm, ILA, ACA, and MOp to LHA. Inset: representative injection sites in LHA for WT and kiFUS mice. White outlines represent LHA boundaries. b Sum of neurons projecting from the selected 28 areas. No difference was detected in the number of neurons projecting to the LHA in WT and kiFUS from the ipsilateral hemisphere (n = 4). c Number of neurons (normalized for total neuronal counts, 50 k) projecting to the LHA from the 28 brain areas in WT and kiFUS. A significant decrease in projections from MOs (P < 0.000 1) and a trend toward decreased projection from ACA (P = 0.163 9) and MOp (P = 0.136 0) were detected (n = 4). d Representative WholeBrain reconstructions of neurons projecting to the LHA in WT and kiFUS mice. A loss of projections from the anterior part of MOs (blue) could be seen (indicated by black arrow). Bars represent mean ± SD. Scale bars, 1 mm. ***P < 0.001