Fig. 4
From: Disruption of orbitofrontal-hypothalamic projections in a murine ALS model and in human patients
Significant misfolded SOD1 burden in the ORBl/vl + AI in mSOD1 mice. a Representative images of misfSOD1+ cells in MOp, SS, ORBl/vl + AI and PL + ORBm; ZsGreen+ neurons projecting to the LHA showed a strong misfSOD1 burden (double-positive) in MOp (arrows). Other structures showed no double-positive neurons (SS, ORBl/vl + AI) or weak mSOD burden (PL + ORBm; arrows). b Burden of misfolded SOD1 (detected by the B8H10 antibody) in MOp, SS, ORBl/vl + AI and PL + ORBm. The ORBl/vl + AI displayed a significantly higher burden than SS or PL (P = 0.001 4 and P = 0.000 6 respectively; areas with unchanged connectivity to LHA) but a significantly lower burden than MOp (P < 0.000 1). Bars show mean ± SD. Scale bars, 50 μm. **P < 0.01, ****P < 0.000 1