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Fig. 2 | Translational Neurodegeneration

Fig. 2

From: Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients

Fig. 2

SOD1 mutation (G93A) induces apoptosis. a Western blot analysis verified the protein levels of Hif1α, Mef2c, Mctp1, Rarb, Bax, and Bcl2 in three different NSC-34 cell lines. b Quantification analysis of western blot. The data represent the average ± SEM of 3 separate experiments. Significantly different at *, p < 0.5; **, p < 0.05. c Hif1α and Mef2c transcripts were increased in mtNSC-34 cells. d Mctp1 and Rarb transcripts were decreased in mtNSC-34 cells e miR-18b (miR-18b-5p) was reduced in mtNSC-34 cells. f LDH releases analysis explained that SOD1 mutation (G93A) induces apoptosis. g Bax mRNAs were upregulated and Bcl2 mRNAs were downregulated in mtNSC-34 cells. Significantly different at *, p < 0.05; **, p < 0.005. The experiments were replicated 3 times

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