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Fig. 6 | Translational Neurodegeneration

Fig. 6

From: Targeting γ-secretase triggers the selective enrichment of oligomeric APP-CTFs in brain extracellular vesicles from Alzheimer cell and mouse models

Fig. 6

D6-treatment abolishes APP-CTF/SorLA interaction and leads to SorLA-CTFα accumulation in the same intracellular compartments than APP-CTFs. a HEK-293 cells stably expressing C99 were transiently transfected with myc-tagged SorlA and treated or not with D6. SorLA was co-immunoprecipitated with α-APP-CTF antibodies only in vehicle treated cells (upper blot). The middle blot corresponds to the lower part of the immunoblot at higher exposure and reveals the presence of SorLA-CTFα in D6-treated cells. The lower blot corresponds to the same protein samples, but blotted with α-APPct, thus showing the levels of immunoprecipitated APP-CTFs. b Immunoblot analysis of SorLAmyc in cell lysates of vehicle- or D6-treated HEK-C99 cells revealed the presence of SorLA-CTFα-myc in D6-treated cells. c Immunocytochemistry of C99 (red) and SorLAmyc (green) in D6-treated co-transfected HeLA cells

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Translational Neurodegeneration

ISSN: 2047-9158

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