Fig. 7
Combination effects of bvPLA2 and STAT3 inhibitor in BV-2 cells. The levels of Aβ1–42 in BV-2 cells were assessed by ELISA (a). The activity of β-secretase in BV-2 cells were investigated by β-secretase activity assay kit (b). The expression of APP and BACE1 in BV-2 cells were detected by Western blotting using specific antibodies (c). The expression of iNOS and COX-2 in BV-2 cells were detected by western blotting using specific antibodies (d). For the cropped images, samples were run in the same gels under the same experimental conditions and processed in parallel. The mRNA expression level of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BV-2 cells treated with bvPLA2 or Stattic were assessed by qRT-PCR (e). Each data representative for three different experiments. Each value is mean ± S.E.M. from 3 samples. ###, Significantly different from control group (p < 0.001). *, Significantly different from bvPLA2 treated group (p < 0.05)