Fig. 6

bvPLA2 directly binds to LD of STAT3. Docking model of bvPLA2 and STAT3 (a). In the enlarged image, purple part means bvPLA2 and green part means STAT3 (a). Schematic domain structures of several forms of STAT3 recombinant proteins (b). Pull-down assay with deletion of binding domains of STAT3 was performed to determine whether bvPLA2 binds to LD of STAT3 (c). Luciferase assay was performed to investigate the effects of bvPLA2 on LD-null STAT3 on LPS-induced STAT3 luciferase activity (d). The mRNA expression levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BV-2 cells transfected with several mutant forms of STAT3 were assessed by qRT-PCR (e). Each data representative for three different experiments. Each value is mean ± S.E.M. from 3 samples. ##, Significantly different from control group (p < 0.01). ###, Significantly different from control group (p < 0.001). *, Significantly different from LPS-treated wild type group (p < 0.05). **, Significantly different from LPS-treated wild type group (p < 0.01). ***, Significantly different from LPS-treated wild type group (p < 0.001)