Fig. 5

Inhibitory effect of bvPLA2 on STAT3 activation. The expression of p-STAT3, STAT3, p-JNK, JNK, p-ERK, ERK, p-p38 and p38 were detected by Western blotting using specific antibodies in the mice brain (a). Each blot is representative for three experiments. For the cropped images, samples were run in the same gels under the same experimental conditions and processed in parallel. Band density was quantified from three mice. *, Significantly different from control group (p < 0.05). **, Significantly different from control group (p < 0.01). The expression of p-STAT3 and STAT3 in BV-2 cells were detected by western blotting using specific antibodies (b). For the cropped images, samples were run in the same gels under the same experimental conditions and processed in parallel. Luciferase assay was carried out to determine the effects of bvPLA2 on LPS-induced STAT3 luciferase activity (c). Each data representative for three different experiments. Each value is mean ± S.E.M. from 3 samples. Whole cell lysate of BV-2 cells were incubated with bvPLA2-conjugated Sepharose 4B. After precipitation, the levels of bound STAT3 were monitored by Western blot analysis (d)