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Fig. 4 | Translational Neurodegeneration

Fig. 4

From: Bee venom phospholipase A2 ameliorates amyloidogenesis and neuroinflammation through inhibition of signal transducer and activator of transcription-3 pathway in Tg2576 mice

Fig. 4

Inhibitory effect of bvPLA2 on LPS-induced neuroinflammation in BV-2 cells. The cell viability in BV-2 cells by bvPLA2 were determined by MTT assay (a). The effect of bvPLA2 on Nitric Oxide production in BV-2 cells were measured by Nitric Oxide assay (b). The expression of iNOS, COX-2, and IBA-1 were detected by Western blotting using specific antibodies in BV-2 cells treated with bvPLA2 (c). β-actin was used as a loading control. For the cropped images, samples were run in the same gels under same experimental conditions and processed in parallel. The mRNA expression level of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and anti-inflammatory cytokines (IL-4, IL-10, and TGF-β) in BV-2 cells treated with bvPLA2 were assessed by qRT-PCR (d). Each data representative for three different experiments. Each value is mean ± S.E.M. from 3 samples. #, Significantly different from control group (p < 0.05). ###, Significantly different from control group (p < 0.001). *, Significantly different from LPS group (p < 0.05). **, Significantly different from LPS group (p < 0.01). ***, Significantly different from LPS group (p < 0.001)

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