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Fig. 2 | Translational Neurodegeneration

Fig. 2

From: Bee venom phospholipase A2 ameliorates amyloidogenesis and neuroinflammation through inhibition of signal transducer and activator of transcription-3 pathway in Tg2576 mice

Fig. 2

Inhibitory effect of bvPLA2 on accumulation of Aβ and factors involved in Aβ production. The accumulation of Aβ plaques in Tg2576 mice brain was determined by Thioflavin S staining (a). The expression of APP and BACE1 were detected by Western blotting using specific antibodies in the mice brain (b). Each blot is representative for three experiments. For the cropped images, samples were run in the same gels under the same experimental conditions and processed in parallel. Band density was quantified from three mice (b). The levels of Aβ1–42 (c) and Aβ1–40 (d) in Tg2576 mice brain were assessed by ELISA. The activity of β-secretase in mice brain was investigated by β-secretase activity assay kit (e). Values measured from each group of mice were calibrated by amount of protein and expressed as mean ± S.E.M. (n = 10). *, Significantly different from control group (p < 0.05). **, Significantly different from control group (p < 0.01). The levels of Aβ1–42 in BV-2 microglial cells were assessed by ELISA (f). The activity of β-secretase in BV-2 cells was investigated by β-secretase activity assay kit (g). Values measured from each group were calibrated by amount of protein and expressed as mean ± S.E.M. (n = 3). The expression of APP and BACE1 in BV-2 cells were detected by western blotting using specific antibodies (h). For the cropped images, samples were run in the same gels under the same experimental conditions and processed in parallel. ##, Significantly different from control group (p < 0.01). ###, Significantly different from control group (p < 0.001). *, Significantly different from LPS group (p < 0.05). **, Significantly different from LPS group (p < 0.01). ***, Significantly different from LPS group (p < 0.001)

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