Fig. 2
RT-QuIC analysis of brain homogenates of patients with PD and neurodegenerative parkinsonisms. a Extraction of soluble and insoluble α-synuclein fractions from brain homogenates of patients with PD, MSA, PSP, CBD, FTDP-17 or NDP control. Western blot analyses confirmed the presence of insoluble α-synuclein only in PD and MSA samples. Blots were immunostained with the AS08 358 antibody. Numbers in the right indicate the position of molecular weights. Asterisks indicate unspecific binding. b RT-QuIC analysis of BH samples. Two μL of sonicated BH collected from PD, MSA, PSP, CBD, FTDP-17 and NDP patients was added to rec-αS substrate and analyzed by means of RT-QuIC. PD and MSA samples efficiently induced rec-αS aggregation that reached higher levels of fluorescence intensities compared to those of PSP, CBD, FTDP-17 and NDP. Average ThT fluorescence intensity was plotted against time. c Assessment of the RT-QuIC detection limits. Serial dilutions (undiluted, 10− 3, 10− 6, 10− 9) of sonicated BH collected from PD, MSA and FTDP-17 subjects were analyzed by means of RT-QuIC. All dilutions efficiently induced rec-αS aggregation but those of FTDP-17 were characterized by lower fluorescence intensities compared to those of PD and MSA. Average ThT fluorescence intensity was plotted against time