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Fig. 1 | Translational Neurodegeneration

Fig. 1

From: Efficient RT-QuIC seeding activity for α-synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy

Fig. 1

RT-QuIC analysis of in vitro generated α-synuclein aggregates. a In vitro generation of α-synuclein aggregates (artificial seeds). rec-αS [140 μM] was induced to aggregate by alternating cycles of incubation and shaking. Average ThT fluorescence intensity was plotted against time; b TEM analysis of final α-synuclein aggregates. Amyloid fibrils were efficiently generated in vitro under well controlled experimental conditions. Scale bar: 500 nm; c Assessment of the RT-QuIC detection limits. Serial dilutions of the artificial seeds previously produced were analyzed by means of RT-QuIC. All dilutions efficiently accelerated the kinetics of rec-αS aggregation. Average ThT fluorescence intensity was plotted against time. Self-assembly refers to unseeded rec-αS reactions

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Translational Neurodegeneration

ISSN: 2047-9158

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