Fig. 1
From: Erythrocytic α-Synuclein as a potential biomarker for Parkinson’s disease
Establishment and characterization of the total α-Syn and pS129 ECL assay systems. a A representative standard curve of the the total α-Syn assay. The detection range was from 5 pg/ml to 10 ng/ml (R2 = 0.999). b Accuracy of the total α-Syn assay was tested by using Linearity-of-dilution, with dilutions of 1:103, 1:104, 1:105 and 1:106 in Diluent 35 in the cytosol and 1:103, 1:104, and 1:105 in the membrane fraction. c Total α-Syn assay accuracy was also tested by spike–in recovery using 100, 200, and 400 pg/ml of unphosphorylated α-Syn monomers. d A representative standard curve of the pS129 assay (Black line, R2 = 0.999). The detection range was from 10 pg/ml to 5 ng/ml. Assay specificity was measured by detecting unphosphorylated α-Syn monomers (red line), or unphosphorylated α-Syn aggregates (blue line) at the same concentrations. e Linearity-of-dilution of the pS129 assay was assessed using dilutions of 1:25, 1:50 and 1:100 in the cytosol and 1:7.5, 1:15 and 1:30 in the membrane fraction. f PS129 assay spike–in recovery was tested by spiking in 250, 500, and 1000 pg/ml of the pS129 standard in the cytosolic fraction and 50, 100, and 200 pg/ml of the standard in the membrane fraction