Fig. 5
Progressive α-synuclein accumulation in the SN of c-rel-/- mice. a-h Representative photomicrographs showing α-synuclein/TH double immunofluorescence labeling in the SN pars compacta of 5-, 7-, 12- and 18-month-old wt and c-rel-/- mice. Please note the stronger and age-related increase of α-synuclein signal in 12- and 18-month-old c-rel-/- mice compared to wt mice. n = 3 animals per group. i, j Representative photomicrographs showing thioflavin-S/α-synuclein double labeling in 12-month-old c-rel-/- mice. The yellow signal in the merge indicates the presence of fibrillary aggregated α-synuclein. n = 3 animals per group. k Nissl-stained sections showing the SN pars compacta. Scale bars: in a = 50 μm for (a-h); in i = 35 μm for (i, j). q = 160 μm. l Evaluation of α-synuclein mRNA transcripts in the SN of 4-, 12- and 18-month-old wt and c-rel-/- mice. Alpha-synuclein expression was increased in c-rel-/- mice at 18 months. n = 3-6 animals per group, *p<0.05 vs. wt mice, t-test