Fig. 7
From: Loss of CREST leads to neuroinflammatory responses and ALS-like motor defects in mice
CREST and FUS Expression Represent Potentially Positive Correlation in CNS
(A and B) Representative confocal immunohistochemistry images (left panels) and intensity quantification (right graphs) of FUS (red) in the cerebral motor cortices (a) and the lumbar spinal cords (b) of Crest −/− (n = 3), Crest +/− mice (n = 3) and WT littermates (n = 3) at P14. Each symbol indicates one image. Top panels show lower magnification, scale bar = 25 μm. Bottom panels show higher magnification of the white square regions of upper panels, scale bar = 10 μm. Error bars represent SEM. *p < 0.05, ***p < 0.001, and ****p < 0.0001, one-way ANOVA. (C and D) Immunoblot (top panels) and intensity quantification (bottom graphs) of protein from the frontal cortices (c) and the lumbar spinal cords (d) of Crest +/− mice (n = 11) and WT littermates (n = 5) at 18 months of age. Relative expression represents the normalized ratios of FUS/Actin (bottom left graphs) or of TDP43/GAPDH (bottom right graphs) band intensities determined by densitometry in Image J. Error bars represent SEM. *p < 0.05, Student’s t test.