Fig. 6
From: Loss of CREST leads to neuroinflammatory responses and ALS-like motor defects in mice
The CREST-BRG1 Complex Represses the Expression of Chemokine Genes via Recruiting Histone Deacetylation Complex in Neurons. a Heatmap showing the differentially expressed genes in primary cortical neurons infected with lentivirus expressing short hairpin RNA targeting CREST (shCREST) or expressing GFP (as control) by microarray analysis (fold change over 2.0). b Radar map showing the gene ontology analysis of gene lists in (A). c Quantitative RT-PCR of two chemokine genes, Cxcl10 and Ccl2, for the confirmation of microarray data in primary cortical neurons transfected with shCREST or GFP as control by lentivirus. (D and E) Quantitative RT-PCR experiments validating the relative mRNA levels of Cxcl10 and Ccl2 in primary cortical neurons isolated from embryotic Crest −/− d and Q394X e mice compared to their WT littermates, respectively. f Chromatin immunoprecipitation on promoter regions of Cxcl10, Ccl2 and c-Fos genes with IgG, anti-CREST or anti-HDAC1 antibody in primary cortical neurons. Signals were normalized as percentage of input. g Quantitative RT-PCR of Cxcl10 and Ccl2 in primary cortical neurons treated with 0.5μM trichostatin A (TSA) for 6h, or infected by lentivirus expressing shRNA targeting BRG1 (shBRG1) or GFP (as control). Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, Student’s t test