Fig. 4
From: Loss of CREST leads to neuroinflammatory responses and ALS-like motor defects in mice
CD68 Staining Confirms Microglial Activation in CREST mutant Mice. (a and b) Representative immunohistochemistry images (a) and quantification (b) of CD68 (green) and Iba1 (red) cells in the cerebral motor cortices of 6-month-old Crest +/− mice (n = 3), 6-month-old Q394X mice (n = 3) and WT mice (n = 3). Each symbol indicates one image, about 5 or 6 images per mouse. Scale bar, 25μm. (C and D) Representative immunohistochemistry images (c) and quantification (d) of CD68 (green) and Iba1 (red) cells in the lumbar spinal cords of 6-month-old Crest +/− mice (n = 3), 6-month-old Q394X mice (n = 3) and WT mice (n = 3). Each symbol indicates one image, about 5 or 6 images per mouse. Scale bar, 25μm. Error bars represent SEM. **p < 0.01 and ****p < 0.0001, one-way ANOVA