Fig. 3
From: Loss of CREST leads to neuroinflammatory responses and ALS-like motor defects in mice
Microglia Are Prevalently Activated in Q394X Mice. a Representative immunohistochemistry images of Iba1-positive microglia (highlighted with arrows, green, upper panels) and their skeletonized appearance (lower panels) in the lumbar spinal cords of 6-month-old Q394X mice (n = 3) and WT littermates (n = 3). Scale bar, 20μm. (b-e) Analysis and quantification of microglial morphological parameters including soma area (b) and roundness (c) of the projection of Iba1-positive cell bodies (each symbol indicating one cell), and branch length (d) and branch number (e) per cell (each symbol indicating one image, about 5 images per mouse) in the lumbar spinal cords of 6-month-old Q394X mice (n = 3) and WT littermates (n = 3). (f) Representative immunohistochemistry images of Iba1-positive microglia (highlighted with arrows, green, upper panels) and their skeletonized appearance (lower panels) in the cerebral motor cortices of 6-month-old Q394X mice (n = 3) and WT littermates (n = 3). Scale bar, 20μm. (g-j) Analysis and quantification of microglial morphological parameters including soma area (g) and roundness (h) of the projection of Iba1-positive cell bodies (each symbol indicating one cell), and branch length (i) and branch number (j) per cell (each symbol indicating one image, about 5 images per mouse) in the cerebral motor cortices of 6-month-old Q394X mice (n = 3) and WT littermates (n = 3). Error bars represent SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, Student’s t test