Fig. 4

GEE upregulates CaMKIV and ERK and increases CaMKIV-CBP binding in the E19 AD embryo. (a-c) GEE increases total and the phosphorylated CaMKIV in the nuclear fraction of E19 embryo brain, as measured by western blotting. (d) GEE increases the association of CaMKIV with CREB-binding protein (CBP), as measured by co-immunoprecipitation. (e) GEE increases CBP phosphorylation, as measured by immunoprecipitation using anti-CBP and western blotting using anti-pSer antibody. Data are presented as the mean ± s.e.m. of at least 3 independent litters of mice, unpaired t-test, *P < 0.05, **P < 0.01 vs. Ctrl