Fig. 2

GEE activates BDNF/TrkB signaling with increases in acetylated histone binding to bdnf gene promoters in AD offspring. (a-d) GEE increases BDNF protein and mRNA levels in 7-m-old offspring hippocampus, as measured by western blotting, immunohistochemical staining (scale bars, 50 μm) and qRT-PCR. (e-g) GEE increases TrkB phosphorylation at Tyr816 without changing the total protein level. (h,i) GEE increases bdnf mRNA transcript variants in 7-m-old offspring hippocampus, as measured by agarose gel electrophoresis. (j,k) GEE increases acetylated histone 4 (H4ac) and H3ac at the indicated bdnf promoter regions, as measured by the CHIP assay. Data are presented as the mean ± s.e.m. of at least 3 independent litters of mice, unpaired t-test with Welch’s correction, *P < 0.05, **P < 0.01 vs. Ctrl