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Fig. 2 | Translational Neurodegeneration

Fig. 2

From: Blocking meningeal lymphatic drainage aggravates Parkinson’s disease-like pathology in mice overexpressing mutated α-synuclein

Fig. 2

LDclns increased intracellular and extracellular aggregation of α-syn in SN of A53T mice. a Representative images showed that α-syn positive intercellular inclusion was increased in TH positive neurons of Ldclns-A53T mice. b Extracellular aggregation of α-syn in adjacent region of laminin-positive microvessels in both WT-LDclns mice and A53T mice, and further increased in A53T-Ldclns mice. c Percentage of α-syn positive area in SN was higher in A53T-LDclns mice than A53T-sham controls (genotype, F(1,12) = 67.8, p < 0.0001; ligament, F(1,12) = 50.39, p < 0.0001; interaction, F(1,12) = 2.684, p = 0.1273). d-f Representative blotting bands and densitometry analysis of α-syn monomer and oligomers (monomer: genotype, F(1,12) = 49.07, p < 0.0001; ligament, F(1,12) = 25.97, p = 0.0003; interaction, F(1,12) = 0.1873, p = 0.6729. oligomers: genotype, F(1,12) = 43.24, p < 0.0001; ligament, F(1,12) = 32.94, p < 0.0001; interaction, F(1,12) = 2.234, p = 0.1608). g ELISA analysis of soluble α-syn from ventral midbrain samples (genotype, F(1,12) = 47.01, p < 0.0001; ligament, F(1,12) = 40.22, p < 0.0001; interaction, F(1,12) = 1.781, p = 0.2068). h ELISA analysis of insoluble α-syn from ventral midbrain samples (genotype, F(1,12) = 76.2, p < 0.0001; ligament, F(1,12) = 28.07, p = 0.0002; interaction, F(1,12) = 4.115, p = 0.0653). Data represent mean ± SEM from 4 mice per group; data in f is from two independent experiments and g-h from three independent experiments

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