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Fig. 1 | Translational Neurodegeneration

Fig. 1

From: Blocking meningeal lymphatic drainage aggravates Parkinson’s disease-like pathology in mice overexpressing mutated α-synuclein

Fig. 1

LDclns blocked peripheral drainage of intracisternal TR-d3 and further reduced TR-d3 influx into brain parenchyma of A53T mice. a Schematic diagram showing injection of TR-d3 into the cisterna magna. b Representative images of TR-d3 fluorescence within superior sagittal (ss) and transverse (ts) meningeal lymphatic vessels of A53T-sham mice and A53T-LDclns mice. LDclns enhanced filling of dural lymphatic vessels. c High magnification micrographs showing that the fluorescent dye was restrictively within Lvey-1 positive lymphatic vessels. d-e Representative images showing TR-d3 fluorescence within the Dclns (d) and midbrain (e) at 30 min after injection into the cisterna magna. f Percentage of TR-d3 fluorescent area in the Dclns (genotype, F(1,12) = 1.739, p = 0.2119; ligament, F(1,12) = 75.13, p < 0.0001; interaction, F(1,12) = 0.7534, p = 0.4024). g Percentage of TR-d3 fluorescent area in the midbrain (genotype, F(1,12) = 32.28, p = 0.0001; ligament, F(1,12) = 41.13, p < 0.0001; interaction, F(1,12) = 0.5650, p = 0.4668). Statistical analysis was performed by Two-way ANOVA followed by Tukey’s post-hoc test. * p < 0.01, ** p < 0.01, *** p < 0.001, Ldclns vs Sham; #p < 0.05, ## p < 0.01, ### p < 0.001, A53T vs WT. Unless otherwise noted, the statistical method is also analyzed the data in Figs. 2-8

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