Skip to main content
Fig. 4 | Translational Neurodegeneration

Fig. 4

From: Constipation, deficit in colon contractions and alpha-synuclein inclusions within the colon precede motor abnormalities and neurodegeneration in the central nervous system in a mouse model of alpha-synucleinopathy

Fig. 4

Intestinal αS distribution in presymptomatic and diseased αS Tg mice. Distribution of αS transgene expression was assessed in young presymptomatic (3 months old) and diseased Tg mice. Fresh mouse intestinal tract was divided in six segments corresponding to anatomical distinct areas: duodenum (D), jejunum (J), proximal ileum (PI), distal ileum (DI), proximal colon (PC), distal colon (DC). NP-40 soluble (N-SOL) and insoluble (N-PEL) fractions were loaded on a SDS-Page and blotted for Syn-1 and α-tubulin or GAPDH antibodies. αS expression was more abundant in the colon of both 3 months old presymptomatic (a) or sick αSTg (d) mice compared to the small intestine (p < 0.0001) or nTg age-matched littermates lysates, although it was present at minimal level in all the fractions examined in Tg mice. Notably insoluble and more surprisingly soluble fraction contained high molecular weight (HMW) αS species. FL αS, full length αS, ΔC αS, truncated αS. b, c, e, f Relative density of αS monomer in soluble (b, e) or insoluble (c, f) fractions. Values on graphs are expressed as % relative to nTg and are given as the mean ± SEM (n = 3–4 per group). * p < 0.05; **** p < 0.0001, two-way ANOVA followed by Fischer’s LSD test

Back to article page