Skip to main content
Fig. 1 | Translational Neurodegeneration

Fig. 1

From: Constipation, deficit in colon contractions and alpha-synuclein inclusions within the colon precede motor abnormalities and neurodegeneration in the central nervous system in a mouse model of alpha-synucleinopathy

Fig. 1

αS Tg presymptomatic mice do not display motor deficits nor αS pathology in the CNS. Young presymptomatic αS Tg at 3 and 6 months were evaluated for αS-driven pathology and motor abnormalities. During the gait test mice were allowed to walk on a white paper strip with painted paws and stance length, sway distance and stride length were measured. The walking pattern in 3 and 6 months old Tg and nTg mice remained unchanged. a Representative walking pattern of presymptomatic 6 months Tgs and nTg littermate do not show differences in stride and stance length and sway distance. b, c, d Graphs of gait parameters analyzed in 3 and 6 months old mice. Values on graphs are expressed as raw data and are given as the mean ± SEM (n = 5/10 per group), two-way ANOVA followed by Fischer’s LSD test. e, f, g, h, i, j Immunofluorescence analysis of pons in presymptomatic (9 months) (ef) or sick (g, h, i, j) αS Tg mice stained with pser129-αS antibody (f, h, I, j) or DAPI (e, g). Phosphorylated pathological αS was found only in sick Tg mice after onset of neurodegeneration, indicating the lack of αS-driven pathology in the CNS of adult presymptomatic animals. Images were acquired with Nikon epifluorescence microscope, objective 4x, scale bar = 100 μm (e, f, g, h) or objective 40x, scale bar = 30 μm (i, j)

Back to article page