Fig. 3

CX3CR1 was mainly located in microglia and was increased in the TG mice at the age of 90 and 120 days. a Double immunofluorescent staining of CX3CR1 (green) and Iba1(red) in the anterior horn of lumber spinal cord of WT and TG mice at the age of 40, 90 and 120 days. Scale bar = 50 μm. b Number of CX3CR1⁺/Iba1⁺ microglia per mm². The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group