Fig. 5
From: Intranasal rapamycin ameliorates Alzheimer-like cognitive decline in a mouse model of Down syndrome
InRapa decreases tau hyper-phosphorylation and expressions of tau kinases. (a 1–8) Representative immunofluorescent images showing tau phosphorylation at Ser416 signal in the CA3 region of the hippocampus from euploid mice treated with Veh and InRapa (a.1–4), and Ts65Dn mice treated with Veh and InRapa (a.5–8). DAPI (blue) was used to identify cell nuclei. Scale bar represent 50 μm. (b) Quantification of fluorescence signal (c) Representative WB showing hippocampal p-tau (Ser416), total tau levels, GSK3β levels and phosphorylation (Tyr216 and Ser9), DYRK1A, CDK5 and RCAN1 protein levels. (d) Quantification of panel C showing levels of tau and p-tau (Ser416)/tau ratio in Eu and Ts65Dn mice treated with InRapa and Veh. (e) Quantification of panel C showing GSK3β, p-GSK3β (Ser9)/GSK3β ratio and p-GSK3β (Tyr216)/GSK3β ratio (f) Quantification of panel C showing levels of RCAN1, DYRK1A and CDK5 total protein levels. Densitometry values shown in the bar graph are the mean of 8 (WB) samples per each group normalized per total load, and given as percentage of Eu Veh, set as 100%