Fig. 5

Forced expression of Lhx8 in AiNPCs enhances cholinergic neuron generation. a Following overexpression of Lhx8, the level of Lhx8 in AiNPCs was determined by qPCR. b The proliferation of AiNPCs after overexpression of Lhx8 was determined by CCK-8 assay. c-h Augmentation of ChAT⁺/Tuj1⁺ cholinergic neuron generation after transduction of Lhx8 in AiNPCs were determined by immunocytochemistry. i The percentage of ChAT⁺/Tuj1⁺ neurons in panels c-h was quantified by counting ChAT⁺ cells and comparing against the total number of Tuj⁺ neurons. ***P < 0.001 by two-tailed t test (n = 3). j Neuronal subtype marker gene expressions in Lhx8-transduced AiNPCs were determined by qPCR. **P < 0.01, ***P < 0.001 by two-tailed t test (n = 3). Scale bars represent 20 μm (c-h). Error bars denote s.d. from triplicate measurements (a, b, i, j)