Fig. 2
From: Direct conversion of mouse astrocytes into neural progenitor cells and specific lineages of neurons
Absence of pluripotency markers in AiNPCs. a-h iPSC and AiNPC cultures were fixed and immunostained for pluripotency markers SSEA-1 and Oct4. i RNA of cultured AiNPCs and control iPSCs was collected and expression of pluripotent-associated genes was analyzed using qPCR. Data were normalized to GAPDH and presented as fold change compared with control iPSCs. Error bars denote s.d. from triplicate measurements. j The Oct4 promoter regulatory region DNA methylation patterns of AiNPCs, control astrocytes, and control NPCs were analyzed using pyrosequencing method. *Human lymphocyte genomic DNA was used as a negative control for pyrosequencing. **Sss1 methyltranferase treated human lymphocyte genomic DNA was used for positive control. Scale bars represent 10 μm (a-h). Error bars denote s.d. from triplicate measurements (i)