Fig. 5
From: Neurodegeneration-associated FUS is a novel regulator of circadian gene expression
The pathogenic R521C mutation of FUS leads to abnormal expression of core circadian genes. a Luciferase activity of transfected PER2-promoter-luciferase in the wild-type (WT) and FUS R521C knock-in (KI) REFs (mean ± s.e.m.; N = 3 experiments; t-test; *:P≤0.05). b mRNA expression levels of Per2 in synchronized REFs (mean ± s.e.m.; N = 6 experiments; two-way ANOVA, *:P≤0.05). c Co-immunoprecipitation of FLAG-GFP, wild-type or R521C-FUS and a FUS C-terminus deletion construct (1-360 residues) with endogenous PSF in HEK293T cells (mean ± s.e.m.; N = 5 experiments; t-test; **:P≤0.01). d Co-immunoprecipitation of endogenous PSF with FUS in the whole brain tissue of the wild-type and FUS R521C knocked-in rats. e Co-immunoprecipitation of FLAG-tagged wild-typed and R518K and P525L FUS with endogenous PSF in HEK293T cells (mean ± s.e.m.; N = 3 experiments, t-test, *:P≤0.05)