Fig. 3
From: Neurodegeneration-associated FUS is a novel regulator of circadian gene expression
FUS regulates the expression of core circadian genes. a, b mRNA expression levels of Per2 (a) and Cry1 (b) in synchronized Neuro-2a cells after siRNA silencing (mean ± s.e.m.; N = 4-6 experiments; two-way ANOVA with Sidak’s multiple comparison test, * represents the significant P-value of overall two-way ANOVA analysis, ***:P≤0.001, # represents the significant P-value of Sidak’s multiple comparison test, #:P≤0.05, ##:P≤0.01, ###:P≤0.001). c Western blot showing the knock-down efficiency of Fus-targeting siRNA (mean ± s.e.m.; N = 3-4 experiments; t-test; ****: P≤0.0001). d, e RT-qPCR showing the mRNA expression of Per2 and Cry1 in cells with indicated transfection conditions in serum shock-synchronized Neuro-2a cells (mean ± s.e.m.; N = 8-9 experiments; One-way ANOVA with Newman-Keuls multiple comparisons test; *:P≤0.05; **:P≤0.01)