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Fig. 5 | Translational Neurodegeneration

Fig. 5

From: Dopaminergic neurons show increased low-molecular-mass protein 7 activity induced by 6-hydroxydopamine in vitro and in vivo

Fig. 5

β5i regulates antigen presentation on DA neurons under 6-hydroxydopamine in vitro. a Chymotrypsin-like proteasome activity on SN4741 cell line was assessed by proteasome activity assays (n = 5 experiments). The expression of β5 and β5i in SN4741 cells at 24 h after the regulation of β5i by RNAi under normal conditions (b, c) and 6-OHDA treatment (d, e). The expression of Bip and TAP1 in SN4741 cells at 24 h after β5i downregulation by RNAi (f, g), and inhibition with PR-957 (h, i). N, normal group without treatment. C, normal condition. M, treated with an empty plasmid. NC, treated with the siRNA negative control. Up, upregulated β5i by overexpression plasmid. Down, downregulated β5i by shRNA. Data are presented as the mean ± SD; n = 4 experiments; * P < 0.05, compared with control; # P < 0.05, compared with the 6-OHDA group; one-way ANOVA

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Translational Neurodegeneration

ISSN: 2047-9158

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