Fig. 2
From: Ultra-High Field Diffusion MRI Reveals Early Axonal Pathology in Spinal Cord of ALS mice
Early axonal structural changes can be observed in spinal cord of ALS mice. a Spinal cord lumbar sections from an YFP, G93A-SOD1 mouse. Regions of Interest (ROI) were obtained from the anterior portion of the spinal cord (SC) white matter (WM) (white square area). Detailed changes in axonal diameter can be seen in the YFP, G93A-SOD1 mice. Note that each WM area in the histology pictures has an approximate voxel size (100 × 100 μm2). Scale bar =1 mm. b Measurements from WM ROIs showed a significant (**p < 0.01) reduction in in axonal areas and an increase in the number of YFP-positive axons (axonal density contained within 100 μm2) in the presymptomatic (P80) YFP, G93A-SOD1 mice. (* = p < 0.05); (** = p < 0.01) (n = 4/5 per group). Nuclear counterstaining with DAPI (blue). Scale bar =10 μm. Abbreviations: WM, white matter; GM, grey matter; YFP, yellow fluorescent protein