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Table 1 Primary and secondary antibodies in double immunostainings

From: Age-dependent alpha-synuclein accumulation and aggregation in the colon of a transgenic mouse model of Parkinson’s disease

Double Immunostaining 1st antibodies 2nd antibodies
α- syn & PGP .5 Anti-α-syn: Santa Cruz Biotechnology, sc-12,767, mouse, dilution: 1/200 Cy3-donkey anti-mouse: Jackson ImmunoResearch, 715–165-150, dilution:1/200
Anti-PGP 9.5: Abcam, ab72910, Chicken, dilution:1/800 Alexa 488 goat anti-chicken: Invitrogen, A11039, dilution: 1/1000
α- syn & α-syn (phosphoS129) Anti-α-syn: Santa Cruz Biotechnology, sc-12,767, mouse, dilution: 1/200 Alexa 488-donkey anti-mouse: Jackson ImmunoResearch, 715–546-151, dilution:1/200
Anti-α-syn (phosphoS129): Abcam, ab51253, rabbit, dilution:1/200 Cy3-donkey anti-rabbit: Jackson ImmunoResearch, 711–165-152, dilution:1/800
α- syn & calretinin Anti-α-syn: Santa Cruz Biotechnology, sc-7011-r, rabbit, dilution: 1/200 Cy3-donkey anti-rabbit: Jackson ImmunoResearch, 711–165-152, dilution:1/800;
Anti-calretinin: Santa Cruz Biotechnology, sc-11,644, goat, dilution:1/400 Alexa 488-donkey anti-goat: Jackson ImmunoResearch, 705–545-147, dilution:1/400
α- syn & nNOS Anti-α-syn: Santa Cruz Biotechnology, sc-12,767, mouse, dilution: 1/200 Alexa 488-donkey anti-mouse: Jackson ImmunoResearch, 715–546-151, dilution:1/200
Anti-neuronal nitric oxide synthase: Abcam, ab76067, rabbit, dilution:1/400 Cy3-donkey anti-rabbit: Jackson ImmunoResearch, 711–165-152, dilution:1/800
α- syn & VIP Anti-α-syn: Santa Cruz Biotechnology, sc-12,767, mouse, dilution: 1/200 Alexa 488-donkey anti-mouse: Jackson ImmunoResearch, 715–546-151, dilution:1/200
Anti-vasoactive intestinal polypeptide: Abcam, ab22736, rabbit, dilution:1/800 Cy3-donkey anti-rabbit: Jackson ImmunoResearch, 711–165-152, dilution:1/800